This mixture is then incubated at fifty five°C for just one hour. Then the sample is phenol-extracted once with phenol/chloroform/isoamyl Liquor Option and centrifuged. The aqueous layer is eliminated to some contemporary microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a next time. Once the pellet is https://www.pinterest.com/pin/993958580252567899/